Anti-hACE2 mouse IgG2b
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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Anti-hACE2-mIgG2b Human ACE2 monoclonal mouse IgG2b antibody |
Show product |
3 x 100 µg |
mabg-hace2-3
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Anti-human ACE2 mouse monoclonal antibody
FACs detection of cellular ACE2 transmembrane receptor
Antibody description
InvivoGen provides a mouse monoclonal antibody (mAb) featuring:
- a variable region that specifically targets the human ACE2 transmembrane protein, established as the SARS-CoV-2 receptor [1]
- the mouse IgG2b constant region
The Anti-hACE2-mIgG2b antibody was obtained following Swiss mice immunization with DNA encoding hACE2. It is produced in hybridoma cells and purified by affinity chromatography with protein A.
Application
- Monitoring cell surface expression of human ACE2 in primary cells or engineered cell lines by FACS (see Figure)
Quality control
- Isotype confirmed by ELISA
- Functionality validated by FACS using HEK-Bue™ hACE2-TMPRSS2 cells
Read our reviews discussing SARS-CoV-2
References
1. Hoffmann M. et al., 2020. SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell. 181:1-16.
Figures
Specifications
Specificity: Target human ACE2 (angiotensin-I-converting enzyme-2)
Clone: 1D10
Source: Hybridoma
Isotype: Murine IgG2b
Formulation: 0.2 μm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents.
Purification: Purified by affinity chromatography with protein A
Quality control:
- The binding of Anti-hACE2-mIgG2b to human ACE2 has been confirmed using Flow cytometry using HEK-Blue™ hACE2-TMPRSS2 cells.
- The murine IgG2b isotype has been confirmed by ELISA.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cellular assays.
Contents
- 3 x 100 µg purified antibody, azide-free, and lyophilized
The product is shipped at room temperature.
Store lyophilized antibody at -20 °C.