Sequencing Primers
Open Reading Frame Vector Sequencing Primers
| pUNO / pUNO1 / pUNO2 / pUNO3 / pSelect | |||
| 5' primer | TGCTTGCTCAACTCTACGTC | Forward | HTLV 5'UTR |
| 3' primer | AACTTGTTTATTGCAGCTT | Reverse | SV40 pAn |
| pLV | |||
| 5' primer | TGGTTACAATGATATACACTG | Forward | rbt ßGlo intron |
| 3' primer | CTCAAGGGGCTTCATGATGTC | Reverse | rbt ßGlo 3'UTR pAn |
Cloning Vector Sequencing Primers
| pCpGfree | |||
| 5' primer | GCAGGAAAAGTGGCACTGTG | Forward | EF1 5'UTR |
| 3' primer | AACTTGTTTATTGCAGCTT | Reverse | SV40 pAn |
| pCpGfree-basic | |||
| 5' primer | GTACCAGTTTTATTGTTTTTAGTGGTAGTG | Forward | ßGlobin MAR |
| 3' primer | CATGGTGGAAGCTACTGTACAC | Reverse | UTR5' |
| pCpGfree-promoter | |||
| 5' primer | GTACCAGTTTTATTGTTTTTAGTGGTAGTG | Forward | ßGlobin MAR |
| 3' primer | GCCATGTGCTCTCTGCCCACTGAG | Reverse | EF1 prom |
| pCpGfree-vitro | |||
| 5' primer | GTGCAGTAGTCTCTGTGAACATTCAAGC | Forward | EF1 prom |
| 3' primer | AACTTGTTTATTGCAGCTT | Reverse | SV40 pAn |
| pCpGrich | |||
| 5' primer | GCAGGAAAAGTGGCACTATG | Forward | EF1 5'UTR |
| 3' primer | AACTTGTTTATTGCAGCTT | Reverse | SV40 pAn |
| pFUSE-CLIg / pFUSE-CHIg | |||
| 5' primer | TGCTTGCTCAACTCTACGTC | Forward | HTLV 5'UTR |
| pFUSE-Fc / pINFUSE (human IgG1, IgG2, IgG3, IgG4 and rabbit IgG) | |||
| 5' primer | TGCTTGCTCAACTCTACGTC | Forward | HTLV 5'UTR |
| 3' primer | CTCACGTCCACCACCACGCA | Reverse | Fc |
| pFUSE-Fc (mouse IgG1) | |||
| 5' primer | TGCTTGCTCAACTCTACGTC | Forward | HTLV 5'UTR |
| 3' primer | GTGAGCACATCCTTGGGCTT | Reverse | Fc |
| pFUSE-Fc / pINFUSE (mouse IgG2a, IgG2b, IgG3) | |||
| 5' primer | TGCTTGCTCAACTCTACGTC | Forward | HTLV 5'UTR |
| 3' primer | GGAAGATGAAGACGGATGGTC | Reverse | Fc |
| pFUSE-Fc (rat IgG1) | |||
| 5' primer | TGCTTGCTCAACTCTACGTC | Forward | HTLV 5'UTR |
| 3' primer | TGGGAGATCAAGAGGATGTC | Reverse | Fc |
| pFUSEN | |||
| 3' primer | AACTTGTTTATTGCAGCTT | Reverse | SV40 pAn |
| pTRIOZ | |||
| Light chain, 5’ primer | TTGCCTCATTCTCAAGCCTCAGAC | Forward | chEF1 5'UTR |
| Light chain, 3’ primer | AACTTGTTTATTGCAGCTT | Reverse | SV40 pAn |
| Heavy chain, 5’ primer | GGGGGAGGGGATGTAATGGCGTTG | Forward | mEF1 5'UTR |
| Heavy chain, 3’ primer | TTAGGCAGAATCCAGATGCTC | Reverse | hßGlobin pAn |
| pVAC1 | |||
| 5' primer | TGTATAGGATGCAACTGCTG | Forward | IL2ss |
| 3' primer | GAAACAAACAGTTCTGAGACCG | Reverse | EF1 pAn |
| pVAC2 | |||
| 5' primer | TCTCAAGCTTCAGACAGTG | Forward | rhEF1 5'UTR |
| 3' primer | GAAACAAACAGTTCTGAGACCG | Reverse | EF1 pAn |
| pVITRO1 | |||
| 5' primer | GAGGCTAATTCTCAAGCCTC | MCS1 Forward | rEF1 5'UTR |
| 3' primer | TCTAGACCTGGAAAGACCAG | MCS1 Reverse | FMDV IRES |
| 5' primer | TTTTGAGCGGAGCTAATTCTCGGG | MCS2 Forward | mEF1 5'UTR |
| 3' primer | AAAAAACCTCCCACACCTCC | MCS2 Reverse | SV40 pAn |
| pVITRO2 / pDUO2 | |||
| 5' primer | TTTTGAGCGGAGCTAATTCTCGGG | MCS1 Forward | mEF1 5'UTR |
| 3' primer | TCTAGACCTGGAAAGACCAG | MCS1 Reverse | FMDV IRES |
| 5' primer | TTGCCTCATTCTCAAGCCTCAGAC | MCS2 Forward | chEF1 5'UTR |
| 3' primer | GCATTCTAGTTGTGGTTTGTCC | MCS2 Reverse | SV40 pAn |
| pVIVO1 | |||
| 5' primer | GCGCGGCTGGAGGTGTGAGG | MCS1 Forward | hGRP94 prom |
| 3' primer | GAAACAAACAGTTCTGAGACC | MCS1 Reverse | EF1 pAn |
| 5' primer | CTCCTGTCGCCCTCAGATCG | MCS2 Forward | hGRP78 prom |
| 3' primer | AAAAAACCTCCCACACCTCC | MCS2 Reverse | SV40 pAn |
| pVIVO2 | |||
| 5' primer | TTTTGAGCGGAGCTAATTCTCGGG | MCS1 Forward | mEF1 5'UTR |
| 3' primer | GAAACAAACAGTTCTGAGACCG | MCS1 Reverse | EF1 pAn |
| 5' primer | TTGCCTCATTCTCAAGCCTCAGAC | MCS2 Forward | chEF1 5'UTR |
| 3' primer | GCATTCTAGTTGTGGTTTGTCC | MCS2 Reverse | SV40 pAn |
| pMONO | |||
| 5' primer | TTTTGAGCGGAGCTAATTCTCGGG | MCS Forward | mEF1 5'UTR |
| 3' primer | TCTAGACCTGGAAAGACCAG | MCS Reverse | FMDV IRES |
Discontinued vectors
| pBLAST42 / pBLAST49 / pORF / pORF9 | |||
| 5' primer | TGCTTGCTCAACTCTACGTC | Forward | HTLV 5'UTR |
| 3' primer | AACTTGTTTATTGCAGCTT | Reverse | SV40 pAn |
| pBLAST45 / pORF5 | |||
| 5' primer | AGCTTTCTTTCCCCAGATCC | Forward | eiF4g 5'UTR |
| 3' primer | AACTTGTTTATTGCAGCTT | Reverse | SV40 pAn |